Attach the DNA Extraction photo from Part A, step 11.
DNA extraction can be used for detecting viruses, police investigations, as well as for diagnostic processes.
To break down the cell wall.
The soap acts as the extraction buffer, helping to release the DNA
To dissolve the other, unnecessary components of the cell.
Proteases are enzymes that break long chain molecules (proteins) into peptides, then those peptides into amino acids.
DNA has a negative charge so it is attracted to the positive pole (attracted to opposites), it is important to separate the DNA from the other molecules in order to get an accurate read.
It is easier to exclude a suspect in a criminal case on the basis of DNA evidence due to the fact that if the DNA markers don’t match then the person is for the most part excluded, but there are a lot of issues that can arise in the collection, the manipulation, and processing of decoding the DNA that it cannot always be 99.99% accurate, and most likely would require multiple samples of DNA to verify that the process was done accurately.
Red blood cells do not contain DNA, but white blood cells do contain DNA which is why it is important that they are present in the sample collected from a crime scene.
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